Immobilization of Pseudomonas Aeruginosa in Different Matrices for the Production of Alkaline Protease
Chandran Masi
Chandran Masi, Department of Biotechnology and Chemical Engineering, College of Biological and Chemical Engg., Addis Ababa Science& Technology University, Ethopia.
Manuscript received on April 02, 2020. | Revised Manuscript received on April 21, 2020. | Manuscript published on May 30, 2020. | PP: 956-959 | Volume-9 Issue-1, May 2020. | Retrieval Number: F9573038620/2020©BEIESP | DOI: 10.35940/ijrte.F9573.059120
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Numerous enzymes are used in various types of industries, and one such enzyme used in several of these industries is proteases. Aforementioned, industries such as dairy, detergent, leather, fermentation and several other industries are benefitted with protease enzyme. In the present study, the efficiency of protease production was studied by enriching and immobilizing several matrices by a gram negative bacteria known as Pseudomonas aeruginosa .The immobilization was carried out by four different matrices under two different concentrations of 3% and 4%.Yielded results revealed highest enzymatic activity of 240 (U/ml) in 3% calcium alginate. Still, second highest enzymatic activity of 230 (U/ml) was seen in 4% calcium alginate. On the contrary, free microbial cells showed an enzymatic activity of 100 (U/ml). The peak activities for other methods area as follows: 4% calcium alginate – 133 (U/ml), 3% agar-agar – 100 (U/ml), 4% agar-agar – 91 (U/ml), 3% Gelatin – 85 (U/ml), 4% Gelatin – 88 (U/ml) and Polyacrylamide – 104 (U/ml). The most optimum matrix for the cellular entrapment of Pseudomonas aeruginosa is seen in 3% calcium alginate for alkaline protease production.
Keywords: Pseudomonas aeruginosa, Immobilization, Enrichment, Alkaline- protease.
Scope of the Article: Production