Preliminary Studies of the Effect of Recognition Layer’s Length in Electrochemical DNA Sensor
Hanis Mohd Yusoff1, Soraya Shafawati Mohamad Tahier2, Ku Halim Ku Bulat3
1Hanis Mohd Yusoff, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
2Soraya Shafawati Mohamad Tahier, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
3Ku Halim Ku Bulat, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Manuscript received on 14 December 2018 | Revised Manuscript received on 26 December 2018 | Manuscript Published on 24 January 2019 | PP: 267-269 | Volume-7 Issue-4S2 December 2018 | Retrieval Number: ES20111017519/19©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: DNA-based electrochemical sensing can promise a simple, accurate and inexpensive for disease diagnosis. One of the main parts in this type of DNA sensor is linker or recognition layer. This study focuses on preliminary approach towards the effect of the linker in DNA sensor. A theoretical approach has been carried out to four different lengths of linker which involve Schiff base molecules with different R group attached ro the molecule (3,6,9, and 12 carbon chain). This study has been carried out using density functional theory (DFT) bu using GAUSSIAN 09 software package with the standard 6-31G9(d,p) basis set for all the calculations. Structure drawing was done with Gauss View 5.0. Energy, dipole moment, HOMO, LUMO, hardness ( ), softness ( ) and energy gap were calculated. Results showed that the length of the linker does not play a role in this study. The best results obtained was from 6 carbon length as it has the highest value of dipole moment which is 4.1622 and the lowest energy gap of 3.13 eV among the other chain. This theoretical result will be compared with experimental results in the near future.
Keywords: DNA Sensor, Linker in Electrochemical DNA Sensor, Density Functional Theory.
Scope of the Article: Pattern Recognition