Superoxide Dismutase-1 Induced Oxidative Stress Mediated Apoptosis in Murine Retinal Pigment Epithelial Cells
T. M. Viswanathan1, A. Arun2, D. Senthil Kumar3, K. Sundar4, T. Kathiresan5
1Thadavarayan Kathiresan, Department of Biotechnology and Chemical Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
2T. M. Viswanathan, Department of Biotechnology and Chemical Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
3A, Arun, Department of Biotechnology and Chemical Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
4D. Senthil Kumar, Department of Biotechnology and Chemical Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
5K. Sundar, Department of Biotechnology and Chemical Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
Manuscript received on 01 December 2019 | Revised Manuscript received on 19 December 2019 | Manuscript Published on 31 December 2019 | PP: 463-466 | Volume-8 Issue-4S2 December 2019 | Retrieval Number: D11541284S219/2019©BEIESP | DOI: 10.35940/ijrte.D1154.1284S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Age related macular degeneration (AMD) is a complicated ocular disease which occurs in elderly people and leads to central vision loss. The AMD generated because of overproduction of oxidative stress which leads to RPE cell death. The present study investigates whether SOD1 induced MRPE cell death based on that overexpression of SOD1 in MRPE cells which induced cell death. The SOD1 gradually increased ROS production and fragmentation of nuclei. To explore the ER stress persuaded UPR via GRP78, and CHOP, protein expression level analyses were carried out by western blotting. Together, our results represent that SOD1 could possibly produce the oxidant induced MRPE cell death.
Keywords: Age-related Macular Degeneration, Caspase, ER Stress.
Scope of the Article: Bio-Science and Bio-Technology