A Novel Biofortification of Selenium Nanoparticles (SeNPs) in Drinking Water and Examination of its Hepatotoxicity in Wister Rats
Subburaman Chandramohan1, Krishnan Sundar2, Azhaguchamy Muthukumaran3
1Subburaman Chandramohan, Department of Biotechnology, Kalasalingam Academy of Research and Higher Education College, Krishnankoil (Tamil Nadu), India.
2Krishnan Sundar, Department of Biotechnology, Kalasalingam Academy of Research and Higher Education College, Krishnankoil (Tamil Nadu), India.
3Azhaguchamy Muthukumaran, Department of Biotechnology, Kalasalingam Academy of Research and Higher Education College, Krishnankoil (Tamil Nadu), India.
Manuscript received on 02 December 2019 | Revised Manuscript received on 20 December 2019 | Manuscript Published on 31 December 2019 | PP: 737-740 | Volume-8 Issue-4S2 December 2019 | Retrieval Number: D11481284S219/2019©BEIESP | DOI: 10.35940/ijrte.D1148.1284S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Biofortification is the delivering micronutrients to populations that may have limited access to diverse diets. Selenium is one of the vital micronutrients that is indispensable to human health and protects the cells by preventing the formation of free radicals. Nanoform of Selenium could be used for biofortification process. In the present work selenium nanoparticles (SeNPs) were synthesized by lemon extract, which acted both as reducing and capping agent. The synthesized SeNPs were characterized by Fourier Transformer InfraRed Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X ray analysis (EDX). FTIR studies revealed the presence of functional groups responsible for nano particle reduction in the nanoparticle formation. The elemental analysis of SeNPs as well as mapping were also carried out which revealed the presence of selenium in the nano suspension. The average size of the synthesized SeNPs was found to be in the range of 200-250 nm with spherical in shape. Furthermore, the prepared SeNPs was fortified in drinking water and given to Wister rats for 21day period, and their effects studied in livers of the Wister rats. The liver histopathology analysis showed that, SeNPs possesses very minimal or no toxicity, when compared to ionic form of selenium given in rats.
Keywords: Selenium Nanoparticles (SeNPs), Sodium Selenite, Biofortification, Wister rat, Histopathology.
Scope of the Article: Bio-Science and Bio-Technology