Characterization of Nanohydroxyapatite isolated from Seer Fish Bones and Waste Scales and its Applications in Lead Removal
Vanavil B1, Yoka Barrathi N S2
1B. Vanavil, Department of Biotechnology, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
2N. S. Yoka Barrathi, Department of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode (Tamil Nadu), India.
Manuscript received on 02 December 2019 | Revised Manuscript received on 20 December 2019 | Manuscript Published on 31 December 2019 | PP: 668-672 | Volume-8 Issue-4S2 December 2019 | Retrieval Number: D11421284S219/2019©BEIESP | DOI: 10.35940/ijrte.D1142.1284S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Hydroxyapatite, (HA), Ca10(PO4)6(OH)2, is recognized as a biomaterial and extensively used for bone implant because of its structural and chemical similarity with mineral components of enamel and human bone. The elements of HA are largely composed of calcium and phosphorus molar ratio of 1.67 and is capable of promoting bone in-growth into prosthetic implant. Huge amounts of by-product waste generated from fish processing industry and market generated an undesirable environmental impact. In this perspective, the present study focused on the isolation and characterization of nanohydroxyapatite (nHA) from bones and waste scales of seer fish. Tissue removal process was carried out by boiling bones and scales at 100°C for 4-5 hours and further boiling with acetone and sodium hydroxide. nHA was isolated by alkaline hydrolysis treatment. The isolated nHA was characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) – Energy Dispersive Spectroscopy (EDS). FT-IR spectrum disclosed the presence of functional groups of HA. SEM-EDS revealed the size shape and elemental composition of the isolated nHA. Further, the application of this isolated nHA in removal of lead was also investigated. At elevated pH levels, nHA adsorbed the lead ions effectively with reduction in lead ions greater than 95%. These results suggest that nHA isolated from bones and waste scales of seer fish can be potentially used for waste water treatment.
Keywords: Alkali Treatment, Lead Removal, Nanohydroxyapatite, Seer Fish.
Scope of the Article: Bio-Science and Bio-Technology