Optimization of Nutrients for the Growth of Scenedesmus Sp. By Response Surface Methodology
Vasumathi K K1, Fenila F2, Nithya E M3, Ramakant Pandey4, Premalatha M5
1Vasumathi K K, Department of Biotechnology, Kalsalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
2Fenila F, Department of Chemical Engineering, Indian Institute of Technology, Bombay (Maharashtra), India.
3Nithya E M, Department of Energy and Environment, National Institute of Technology Tiruchirappalli (Tamil Nadu), India.
4Ramakant Pandey, Department of Energy and Environment, National Institute of Technology, Tiruchirappalli (Tamil Nadu), India.
5Premalatha M, Department of Energy and Environment, National Institute of Technology, Tiruchirappalli (Tamil Nadu), India.
Manuscript received on 02 December 2019 | Revised Manuscript received on 20 December 2019 | Manuscript Published on 31 December 2019 | PP: 664-667 | Volume-8 Issue-4S2 December 2019 | Retrieval Number: D11411284S219/2019©BEIESP | DOI: 10.35940/ijrte.D1141.1284S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Microalgae, the photosynthetic microorganisms growing abundantly in various ecosystems are not just weeds but a potential source for sequestration of CO2. Biological sequestration of CO2 by microalgae is much more significant from the physical and chemical methods as it produces biomass (source of biofuels) apart from sequestering CO2.The growth of microalgae depends upon and requires optimum concentration of light intensity, temperature, nutrients, concentration of inoculum to produce higher yield of biomass as well its byproducts. The present study aims to optimize the nutrients using RSM for growth of microalgae, Scenedesmus sp, which was isolated locally. The other parameters such as light intensity (160 µE/m2 /s), temperature (250 C) and light/dark cycle (12/12) were maintained constant to reveal the nutrient effect on the biomass growth. The nutrients studied were nitrate (urea (0-500 ppm)), phosphate (potassium dihydrogen phosphate (0-500 ppm)), and bicarbonate ((potassium hydrogen carbonate (0-2000 ppm)). Face Centered Central Composite Design (FCCD) of 15 runs was obtained using Design Expert 8.0.7.1 (Trail version). The higher yield of biomass was obtained at optimized nutrient concentrations of 0 ppm nitrate, 250 ppm phosphate, and 1000 ppm bicarbonate. The response equation (biomass yield) as a function of nitrate, phosphate and carbonate concentrations was obtained which helps to identify the effect of various composition. The RSM technique will help to identify the best combination of nutrients for the growth of Scenedesmus sp.
Keywords: Carbonate, Nitrate, Phosphate, FCCD Optimization.
Scope of the Article: Bio-Science and Bio-Technology