Evaluation of in Vitro Anticancer Activity of Crude Extracts Obtained from Two Bacterial Strains Isolated from Kodiyampalayam, Tamil Nadu
S Satheesh Kumar1, Uma C2, Illiyas Maqbool Malla3, K Muthulakshmi4, P Sivagurunathan5
1Satheesh Kumara, Department of Microbiology, Annamalai University, Chidambaram (Tamil Nadu), India.
2Uma Ca, Department of Microbiology, Annamalai University, Chidambaram (Tamil Nadu), India.
3K Muthulakshmia, Department of Microbiology, Annamalai University, Chidambaram (Tamil Nadu), India.
4P Sivagurunathana, Department of Microbiology, Annamalai University, Chidambaram (Tamil Nadu), India.
5Illiyas Maqbool Mallab, Department of Biochemistry & Biotechnology, Annamalai University, Chidambaram (Tamil Nadu), India.
Manuscript received on 19 November 2019 | Revised Manuscript received on 04 December 2019 | Manuscript Published on 10 December 2019 | PP: 291-295 | Volume-8 Issue-3S2 October 2019 | Retrieval Number: C10561083S219/2019©BEIESP | DOI: 10.35940/ijrte.C1056.1083S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Extracts of natural products, especially microorganisms, have been a valuable source of various molecules in many drug discovery efforts and led to the discovery of many important drugs. The identification of microbial strains with promising biological activities and the purification of biomolecules responsible for activities led to the discovery of many biologically active molecules.Crude extracts of two marine bacterial isolates isolated from marine sediment samples, were studied for their in vitro anticancer activity against human oral squamous carcinoma (KB) cell line. Morphological studies and biochemical tests of the two bacterial extracts were also carried out. Cytotoxic, intracellular ROS, nuclear staining and apoptotic morphological alteration studies were carried out to assess the anticancer potential of each extract. The crude ethyl acetate extract of KP-9 isolate showed promising results by MTT assay with IC50 as low as 7.9 μg/ml while as KP-7 showed an IC50 value of 21.1μg/ml in KB cells. The crude extract of KP-9 augmented higher levels of ROS and displayed higher potential by inducing higher levels of nuclear and morphological alterations when compared with KP-7 bacterial extract in KB cells.
Keywords: Anticancer Activity, Microbial Extracts, Cytotoxicity, ROS.
Scope of the Article: Performance Evaluation of Networks