Evaluation of Occupational Exposure to Xylene in Petroleum Workers by Assessing Urinary Methyl Hippuric Acid and Cellular Changes of Exfoliated Epithelial Cells in Buccal Mucosa Smears
Mohamad Ayas1, Vidya Gowdappa Doddawad2, BM Gurupadayya3
1Mohamad Ayas Post Graduate Students, Department of Oral Pathology & Microbiology, JSS Dental College and Hospital, A Constituent College of JSSAHER University, Mysuru, Karnataka, India.
2Vidya Gowdappa Doddawad, Associate Professor, Department of Oral Pathology & Microbiology, JSS Dental College and Hospital, A Constituent College of JSSAHER University, Mysuru, Karnataka, India.
3BM Gurupadayya, Professor Department of Pharmaceutical Chemistry, JSS College of Pharmacy, A Constituent College of JSSAHER University, Mysuru, Karnataka, India.
Manuscript received on 20 March 2019 | Revised Manuscript received on 25 March 2019 | Manuscript published on 30 July 2019 | PP: 1323-1328 | Volume-8 Issue-2, July 2019 | Retrieval Number: B1946078219/19©BEIESP | DOI: 10.35940/ijrte.B1946.078219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Xylene is an important component of petrol and a widely distributed environmental contaminant. About 98% of Xylene is derived from the petrochemical and petroleum refining industries. When human get expose to xylene, which is one of the major Geno toxicants, may be associated with a range of acute/chronic diseases and cancer still literature is not available. Taking into our mind that occupational exposure to such derivatives may possess genotoxic risk or not. Hence our study aims to investigate and correlate the cellular changes in exfoliated oral epithelial cells oral smears with urinary methyl hippuric acid level estimation in petrol pump workers and to identify the significant role of xylene on oral mucosa.
Materials and Methods: Urine samples and oral buccal mucosa smears were collected from 30 healthy individual (control) and 30 petroleum pump workers (case) working in petroleum station who are above 18 years of age. The urine was collected before exposure/work shift and after completion of work shift. The urinary methyl hippuric acid (MHA) level was analyzed by using Shimadzu UV-Visible Spectrophotometer procedure. The smeared slides were stained with PAP stain and analyses the cytomorphometric changes of exfoliated epithelial cells by using Axio Vision SE64 Rel 4.9.1. Ink Software.
Keywords: Exfoliated Buccal cells, Urine Methyl Hippuric Acid level, UV Spectrophotometry, Xylene
Scope of the Article: Petroleum and Mineral Resources Engineering