Some Observations on Thin Layer Chromatography Technique
Mudiganti Ram Krishna Rao1, Sampad Shil2
1Mudiganti Ram Krishna Rao, Professor, Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai.
2Sampad Shil, Student, Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai.
Manuscript received on 20 March 2019 | Revised Manuscript received on 26 March 2019 | Manuscript published on 30 July 2019 | PP: 1700-1702 | Volume-8 Issue-2, July 2019 | Retrieval Number: B1010078219/19©BEIESP | DOI: 10.35940/ijrte.B1010.078219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The present study is to report the various problems faced during TLC methodology. Although used regularly some technical aspects must be kept in mind to get better and uniform results. During our experiments with TLC methods we came across some problems and here these aspects of TLC methodology are being highlighted. It is suggested that the solvent use as mobile phase should also be used for extraction of any particular phytochemical. TLC plates should be 3 to 4 mm thick, to be dried for at least 72 hrs. It is also suggested that potassium permanganate solution gives better clarity in visualizing the spots.
Key words: TLC, Extraction. Methodology, Phytochemical, Potassium Permanganate
Scope of the Article: Bio-Science and Bio-Technology