Myostatin mRNA Expression and its Association with Carcass and Body Weight of Local Pigs from the Islands in North Sulawesi, Indonesia
Revolson Alexius Mege1, Yermia Samuel Mokosuli2, Nonny Manampiring3, Debby Rayer4, Friska Mery Montolalu5
1Revolson Alexius Mege, Biology Department, Faculty of Mathematics and Science, Manado State University, Indonesia.
2Yermia Samuel Mokosuli, Biology Department, Faculty of Mathematics and Science, Manado State University, Indonesia.
3Nonny Manampiring, Biology Department, Faculty of Mathematics and Science, Manado State University, Indonesia.
4Debby Rayer, Biology Department, Faculty of Mathematics and Science, Manado State University, Indonesia.
5Friska Mery Montolalu, Department of Pharmacy, University of Trinita Manado, Indonesia.
Manuscript received on 24 June 2019 | Revised Manuscript received on 07 July 2019 | Manuscript Published on 17 July 2019 | PP: 18-23 | Volume-8 Issue-2S July 2019 | Retrieval Number: B10040782S19/2019©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Myostatin gene is known as a member of the growth gene’s superfamily (TGF-β) which works to suppress the muscle growth. This study was designed to investigate the Myostatin mRNA expression and its association with body weight and carcass of local pigs from the islands in North Sulawesi.The parameters measured were Myostatin mRNA expression by reverse tranriptase RT-PCR, body weight, and carcass weight of local pigs from the islands in North Sulawesi. mRNA sample is taken from sceletal muscle of sacrifice pigs. The Myostatin primer gene used is F = 5 ‘CCA CTC CGG GAA CTG ATT GA 3’ and R = 5 ‘TCT CA 3 AGG AGT CTT GAC GGG’ with its housekeeping gene GAPDH.The results showed that myostatin mRNA expression was correlated (P <0.05) with body weight and carcass weight of local pigs from the islands in North Sulawesi. Myostatin regulates the carcass and growth performance. Myostatin mRNA expression was correlated with carcass and body weight of local pigs from the islands in North Sulawesi. The expression of the myostatin gene can be used as a cheap selection model and can be done in a shorter time, especially to select quality livestock breeds.
Keywords: Body Weight, Carcass, Local Pigs, Mrna Expression, Myostatin. Gene Livestock Breeds.
Scope of the Article: Cryptography and Applied Mathematics