Detection of Serine Protease Gene (Ser-P) In Marine Isolates of Aeromonas Hydrophila
S. Selvakumar1, Umer Mushtaq Dar2, Illanchezian Seetha lakshmi3
1Dr. S. Selvakumar, Ph.D., Associate Professor, Department of Industrial BioTechnology, Bharath University, Chennai, India.
2Umer Mushtaq Dar, Dept. of IBT, Bharath Institute of Higher Education and Research, Chennai-600073, India.
3Illanchezian Seetha lakshmi, Dept. of IBT, Bharath Institute of Higher Education and Research, Chennai-600073, India.
Manuscript received on 01 April 2019 | Revised Manuscript received on 05 May 2019 | Manuscript published on 30 May 2019 | PP: 49-51 | Volume-8 Issue-1, May 2019 | Retrieval Number: A1399058119/19©BEIESP
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Abstract: Marine water samples were collected from marina beach in Chennai for screening the isolation of serine protease gene from Aeromonas hydrophila by using PCR analsis.The. Samples were collected and analysed the genomic DNA of Aeromonas hydrophila was isolated. Before analysing polymerase chain reaction with the selected isolates each primer set was tested. Polymerase chain reaction was performed to screen the ser-P genes. This study showed that PCR detection may prove to be an vital tool for the detection, identification, differentiation and distribution of serine protease genes in organisms. This polymerase chain reaction technique will give scientists an alternative way to find out the nature of pathogenicity in Aeromonas species and their distribution in isolates from different sources. Our present study clearly indicate that the PCR analysis detects serine protease genes in Aeromonas hydrophila species by using a pair of primers for gene.
Key words: Aeromonas Hydrophila, Environment, Gene, PCR, Serine Protease.
Scope of the Article: Bio – Science and Bio – Technology