Callus Induction of Justicia Gendarussa Leafexplant (Justicia Gendarussa Burm.f.) with Growth Regulator 2,4-D, IBA and Kinetin
Dwi Kusuma Wahyuni1, Nur Rohmatin2, Bambang Prajoga3, Eko Wardoyo4, Junairiah5, Hery Purnobasuki6
1Dwi Kusuma Wahyuni, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
2Nur Rohmatin, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
3Bambang Prajoga, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
4Eko Wardoyo, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
5Junairiah, Faculty of Science and Technology, Airlangga university, Surabaya, Indonesia.
6Hery Purnobasuki, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
Manuscript received on 25 March 2019 | Revised Manuscript received on 04 April 2019 | Manuscript Published on 27 April 2019 | PP: 452-456 | Volume-7 Issue-6S2 April 2019 | Retrieval Number: F10560476S219/2019©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The Justicia Gendarussa (Justicia Gendarussa Burm.f.) is an Indonesian medicinal plant which contains a flavonoid compound that is act as antifertility. The study aimed to understand the effect of growth regulator 2,4-D, Indole Butyric Acid (IBA) and kinetin toward the callus induction from Justicia Gendarussa leaf explant. This study was used factorial design with 24 combinations and 3 replications. The basic medium used were Murshige and Skoog (MS) medium with growth regulator 2,4-D and IBA (0.5 mg/l; 1mhg/l; 1.5mg/l) with kinetin (0.5mg/l; 1mg/l; 1.5mg/l; 2mg/l). The data analysis was used two ways multivariate Anova and Duncan test. The result showed growth regulator 2,4-D with kinetin had longest time of the formation on 5th ,6th and 7th days with 100% callus formation. The best weight of callus with growth regulator 2,4-D with 1.5 mg/l and 1.5 mg/l of kinetin (1.8147 ±0.1147 g), while best dry weight of callus with 1 mg/l of growth regulator 2,4-D with 0.5mg/l (0.1240 ±0.0277 g) which callus had brown colour and crust texture. Meanwhile, the combination of IBA with kinetin had longest time of callus formation was at 6th and 7th day with 100% callus formation. The highest weight loss of callus in growth regulator with IBA 1mg/l with 1.5 mg/l of kinetin (0.4688±0.0958 g), while best callus dry weight in IBA 1 mg/l with 2 mg/l of kinetin (0.0895 ± 0.0089 g) with brown callus colour and compact texture. These results had proved growth regulator 2,4-D with kinetin and IBA with kinetin was able to induce callus on Justicia Gendarussa leaf explant.
Keywords: Justicia Gendarussa Callus Induction Growth.
Scope of the Article: Social Sciences