Calcium Phosphate /Poly (Ethylene Glycol) Bone Cement: Cell Culture Performance
Sufiamie Hablee1, Nurhusna Samsudin2, Iis Sopyan3, Maizirwan Mel4, Hamzah Mohd. Salleh5, Md. Mujibur Rahman6, Yumi Z. H-Y Hashim7, Raha Ahmad Raus8
1Sufiamie Hablee, Department of Manufacturing and Materials Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
2Nurhusna Samsudin, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
3Iis Sopyan, Department of Manufacturing and Materials Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
4Maizirwan Mel, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
5Hamzah Mohd. Salleh, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
6Md. Mujibur Rahman, Department of Mechanical Engineering, College of Engineering, Universiti Tenaga Nasional (UNITEN), Selangor, Malaysia.
7Yumi Z. H-Y Hashim, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
8Raha Ahmad Raus, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), Malaysia.
Manuscript received on 21 March 2019 | Revised Manuscript received on 02 April 2019 | Manuscript Published on 18 April 2019 | PP: 82-86 | Volume-7 Issue-6S March 2019 | Retrieval Number: F02190376S19/2019©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Calcium phosphate cement (CPC) for injectable bone cement application has been developed in this study. The CPC was produced using a novel wet chemical precipitation method derived hydroxyapatite (HA) powder. The calcium and phosphorus precursors used to synthesize HA powder were calcium hydroxide, Ca(OH)2 , and di-ammonium hydrogen phosphate, (NH4 )2HPO4 . The HA powder was mixed with distilled water at certain powder-to-liquid (P/L) ratios. In this study, the P/L ratios were varied at 1.3 and 1.7. PEG was added into CPC with the P/L ratio of 1.3, and it was adjusted at 1 and 5 wt%. The results of this study revealed that higher P/L ratio contributed to the decreased in porosity of CPC. Meanwhile, the addition of PEG increased the porosity of CPC. This is significant for cells adhesion and proliferation, such that cell proliferate faster and better adhesion with the incorporation of PEG into CPC. The cell culture on CPC has proven that the fabricated CPC shows no toxic reaction and cells grow well.
Keywords: Calcium Phosphate Cement; Injectable; Polymeric Additive; Vero Cell Culture; Wet Chemical Precipitation.
Scope of the Article: Mechanical Maintenance