HPLC Characterization and Assessment of Antioxidant Status of Vetiveria Zizanioides Roots
Punithavathi Manogaran1, Suriyavathana Muthukrishnan2, Kavitha Rani Mari3, Anandhi Eswaran4
1Punithavathi Manogaran, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
2Kavitha Rani Mari, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
3Anandhi Eswaran, Ph.D Research Scholars, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
4Dr. Suriyavathana Mthukrishnan, Associate Professor, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
Manuscript received on 19 September 2019 | Revised Manuscript received on 06 October 2019 | Manuscript Published on 11 October 2019 | PP: 482-485 | Volume-8 Issue-2S10 September 2019 | Retrieval Number: B10830982S1019/2019©BEIESP | DOI: 10.35940/ijrte.B1083.0982S1019
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Vetiveria zizanioides has been assigned for the extraction of phenolic acids and flavonoids for soluble, glycoside and wall-bound fractions. There was the largest number of phenolic acids and flavonoids in the methanolic extract that constitutes the cell wall-bound portion. Free radicals can induce biomolecules to oxidize, resulting in cell damage and countless illnesses. The present study investigates the role of enzymatic antioxidants, i.e. catalase, superoxide dismutase, glutathione peroxidase,glutathione reductase.Vitamin Eand C enzyme activity was nonenzymaticantioxidant action by spectrophotometric method. The enzymatic glutothi-one peroxidase antioxidant was found to be exampling than the rest while Vitamin E, notified found to be best activity ratherthan Vitamin C.The reversed highperformance liquid chromatographic techniquewas created and validated for the concurrent identification of free phenolic acids and flavonoids using a photodiode array detector with gradient elution.(Caffeicacid, Hydroxy benzoic acid, Rutin, Quercetin, Para- cumaric acid and Kaempferol) in the methanolic root extract of Vetiveria zizanioides.
Keywords: Vetiveria Zizanioides, Enzymatic Antioxidants, Non-enzymatic Antioxidants, HPLC Analysis.
Scope of the Article: Bio-Science and Bio-Technology