Expression of Nitrite Transporter Gene to Increase Nitrate Absoprtion in Rice Plant (Oryza Sativa Cv Nipponbare) as Sole Nitrogen Source
Sustiprijatno
Sustiprijatno, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Indonesian Agency for Agricultural Research and Development, Jalan Cimanggu, Bogor, Indonesia.
Manuscript received on 03 August 2019 | Revised Manuscript received on 26 August 2019 | Manuscript Published on 05 September 2019 | PP: 176-178 | Volume-8 Issue-2S7 July 2019 | Retrieval Number: B10430782S719/2019©BEIESP | DOI: 10.35940/ijrte.B1043.0782S719
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Incorporated of CsNitr1-L-transgene in rice plant was showed to active and transport NO2 – from cytosol into chloroplast. With a PCR method screening of CsNitr1-L gene were confirmed which the CsNitr1-L-transgene have amplification band, while the plant were not contained CsNitr1-L gene, there are no amplification band. Some strain were obtained and got a seeds. Almost CsNitr1-L-transgene was raising in all parameter test, such as plant height, tillers number, respectively. The accumulation of NO2 – in the leaves of CsNitr1-L-transgene was lower compared with wild type. CsNitr1-L is a cucumber NO2 – -transporter gene that located in chloroplast membrane have been transformed into japonica rice cv. Nipponbare. A series experiments was carried out to understand the expression- CsNitr1-L-transgenic rice. The seeds were cultivated to observe the activity of CsNitr1-L-transgene with medium containing NO3 – or NO2 – as inducer. Agronomical test, like tillers number and plant height were observed and compare with non CsNitr1-L- transgenic rice. While the NO2 – content in leaves were analyzed with capillary electrophoresis method.
Keywords: Nitrite Transporter, Rice, Transgenic, Nitrate, Nitrite.
Scope of the Article: Social Sciences